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Illumina Inc humanht 12v 3 illumina sentrix beadchip microarrays
Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina <t>microarrays.</t> Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.
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Intercytex allogeneic neonatal fibroblasts vaveltatm
Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina <t>microarrays.</t> Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.
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Qiagen rneasy mini kit
Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina <t>microarrays.</t> Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.
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Qiagen rneasy plus mini kit
Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina <t>microarrays.</t> Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.
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Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina microarrays. Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.

Journal: Scientific Reports

Article Title: Hepatic lipid metabolism is altered in Ubiad1 +/− mice of both sexes

doi: 10.1038/s41598-025-91283-7

Figure Lengend Snippet: Ubiad1 expression associates with subcutaneous adipose tissue weight. ( A , B ) Locus plot showing the associations between single nucleotide polymorphisms (SNPs) and subcutaneous adipose tissue Ubiad1 expression on chromosome 4 in ( A ) male and ( B ) female HMDP mice. The significant associations between SNPs and Ubiad1 mRNA expression levels in subcutaneous white adipose tissue (WAT) are depicted as dots and refer to the p-value of the association (-log 10 of p-value), and location on chromosome 4. ( C , D ) Ubiad1 mRNA expression is significantly correlated with subcutaneous WAT mass in ( C ) male and ( D ) female mice. ( E , F ) Pathway enrichment analysis using the top 500 genes that were strongest correlated with Ubiad1 mRNA expression levels in the HMDP of ( E ) male, and ( F ) female mice. GO Biological process (GO BP) pathways were performed with positively correlated mRNAs (red) and negatively correlated mRNAs (blue) individually. The top 10 enriched pathways are presented. X-axis: P-value (-log10) of enriched terms. ( G , H ) Fold changes of UBIAD1 mRNA expression in the ( G ) adipocyte fraction and ( H ) stromal vascular fraction (SVF) of subcutaneous WAT of lean or obese human subjects. UBIAD1 mRNA was measured by Illumina microarrays. Data were normalized against the average UBIAD1 expression of lean individuals. Data in graphs are shown as means ± SD (n = 12 per group). P-values were calculated with unpaired t test. **p < 0.01. ( I ) Pathway enrichment analysis of the mRNAs that correlated strongest (366 positively and 264 negatively correlated; correlation coefficient >0.6) with UBIAD1 mRNA expression levels in adipocyte from subcutaneous WAT. PANTHER GO-Slim Biological Process pathway analyses were performed individually with positively correlated mRNAs (red) and negatively correlated mRNAs (blue). The top 10 enriched pathways are presented. X-axis: p-value (-log10) of enriched terms.

Article Snippet: For gene expression analysis, RNA with high integrity was extracted from the adipocytes and stromal vascular fraction and converted to biotin-labeled cRNA, with subsequent hybridization of the cRNA to HumanHT-12v.3 Illumina Sentrix BeadChip microarrays before analysis in the Illumina iScan system (Illumina Inc., San Diego, CA, US).

Techniques: Expressing